We do research in an interdisciplinary field of Sensing Technology, Nano-Biotechnology, and Material Science to provide pertinent solutions to healthcare
The lab research-interest focuses on the vivid fields of Medical Device Development technology, BioNanoSensors, NanoDiagnostics, NanoAnalytics, Pre-formulation and Formulation of Smart-materials & their applications
We develop medical devices for diverse disease diagnostics and therapeutics. The devices that are affordable to the mass population, easy-to-use, and portable, will be made available to be used in remote or resource-limited settings.
Our lab has expertise in the development of lateral flow immunoassays and portable electronic devices required for signal processing and analysis. Sensor-based colorimeter, as well as the fluorimeter, have been developed earlier
Our lab works on the synthesis of various nanoparticles including semiconductor nanocrystals i.e. Quantum Dots. The biofunctionalization of nanoparticles improves its utility in the development of biosensors.
Research supported by:
In this work, we demonstrate label-free electrochemical impedance spectroscopy (EIS) based alkaline phosphatase (ALP) detection using gold nanoparticles (AuNPs), electrochemically engineered Au-nano-Dendroids, and graphene oxide (GO) nanocomposite. These nanomaterials were sequentially deposited on to the screen-printed carbon electrode (SPCE) and antibodies against ALP (anti-ALP) were immobilized using carbodiimide bioconjugation process. The sensor probe has been characterized extensively using TEM, EDX, SAED, XRD, FE-SEM, FTIR, DIC, and electrochemical techniques. The analytical performance of fabricated biosensor has been evaluated using EIS, where linear dynamic range and limit of detection were obtained to be 100–1000 U/L and 9.10 (±0.12) U/L, respectively. The developed biosensor showed high selectivity towards ALP with negligible interference (ksel « 1; n = 3) due to coexisting molecules. The sensor probe has successfully recovered ALP between 108.84% and 172.50% (n = 3) in human serum samples. The sensor has been used to estimate ALP in clinical serum samples, where the level was found to be 83.15 U/L and was comparable with standard technique used in the hospitals. The shelf life, stability, and reproducibility have also been evaluated.
— Kuldeep Mahato, Buddhadev Purohit, Ashutosh Kumar, Pranjal Chandra*